4NDZ

Structure of Maltose Binding Protein fusion to 2-O-Sulfotransferase with bound heptasaccharide and PAP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Maltose-binding periplasmic protein, Heparan sulfate 2-O-sulfotransferase 1 fusion	polymer	658	74402.5	6	UniProt (P0AEX9) UniProt (Q76KB1) Pfam (PF01547) Pfam (PF03567) In PDB	Escherichia coli (bantam chickens)
2	G, I, L, N, P	beta-D-glucopyranuronic acid-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid	branched		1408.1	5	In PDB GlyTouCan (G54413LX)
3	H, J, K, M, O	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	5	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	A, B, C, D, E...	ADENOSINE-3'-5'-DIPHOSPHATE	non-polymer		427.2	6	Chemie (A3P)		cHS2ST
5	A, B, D, E	P-NITROPHENOL	non-polymer		139.1	4	Chemie (NPO)

Sequence modifications

A, B, C, D, E, F: 1 - 367 (UniProt: P0AEX9)

PDB	External Database	Details
Ala 359	Glu 385	engineered mutation
Ala 362	Lys 388	engineered mutation
Ala 363	Asp 389	engineered mutation
Asn 367	Arg 393	engineered mutation
Ala 368	-	linker
Ala 369	-	linker
Ala 370	-	linker

A, B, C, D, E, F: 1069 - 1356 (UniProt: Q76KB1)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	446414.9
Branched	Number of molecules	10
	Total formula weight	8752.2
Non-Polymers*	Number of molecules	10
	Total formula weight	3119.6
All*	Total formula weight	458286.7

*Water molecules are not included.