4ND6

Crystal structure of apo 3-nitro-tyrosine tRNA synthetase (5B) in the open form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tyrosine-tRNA ligase	polymer	314	36073.8	1	UniProt (Q57834) Pfam (PF00579)	Methanocaldococcus jannaschii	Tyrosyl-tRNA synthetase, TyrRS
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	C (A)	water	water		18.0	125	Chemie (HOH)

Sequence modifications

A: 1 - 306 (UniProt: Q57834)

PDB	External Database	Details
His 32	Tyr 32	engineered mutation
Cys 70	His 70	engineered mutation
Asn 75	Gln 75	engineered mutation
Ser 158	Asp 158	engineered mutation
Ala 159	Ile 159	engineered mutation
Arg 162	Leu 162	engineered mutation
Leu 307	-	expression tag
Glu 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36073.8
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	36165.9

*Water molecules are not included.