4N6N
Crystal structure of oxidized legumain in complex with cystatin E/M
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Legumain | polymer | 278 | 31689.6 | 1 | UniProt (Q99538) Pfam (PF01650) In PDB | Homo sapiens (human) | Asparaginyl endopeptidase, Protease, cysteine 1 |
2 | B | Cystatin-M | polymer | 131 | 14924.9 | 1 | UniProt (Q15828) Pfam (PF00031) In PDB | Homo sapiens (human) | Cystatin-6, Cystatin-E |
3 | C | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
4 | D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
5 | A | IODIDE ION | non-polymer | 126.9 | 5 | Chemie (IOD) | |||
6 | water | water | 18.0 | 244 | Chemie (HOH) |
Sequence modifications
A: 26 - 303 (UniProt: Q99538)
B: 4 - 124 (UniProt: Q15828)
PDB | External Database | Details |
---|---|---|
Gln 263 | Asn 263 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 2 | - | expression tag |
Asp 3 | - | expression tag |
Leu 125 | - | expression tag |
Glu 126 | - | expression tag |
His 127 | - | expression tag |
His 128 | - | expression tag |
His 129 | - | expression tag |
His 130 | - | expression tag |
His 131 | - | expression tag |
His 132 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 46614.6 | |
Branched | Number of molecules | 2 |
Total formula weight | 1010.9 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 634.5 | |
All* | Total formula weight | 48260.0 |