4N5S
Ternary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant (Cs3C KlenTaq) with DNA and ddCTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | 5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3' | polymer | 12 | 3617.4 | 1 | |||
| 2 | B (C) | 5'-D(*AP*A*AP*GP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3' | polymer | 16 | 4964.2 | 1 | |||
| 3 | C (A) | DNA polymerase I, thermostable | polymer | 553 | 62434.8 | 1 | UniProt (P19821) | Thermus aquaticus | Taq polymerase 1 |
| 4 | D, F, G, H, I... (B, A) | MANGANESE (II) ION | non-polymer | 54.9 | 6 | Chemie (MN) | |||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | K (A) | 2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE | non-polymer | 451.2 | 1 | Chemie (DCT) | |||
| 7 | L (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 8 | M, N (A) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 9 | O (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 10 | P, Q, R (B, C, A) | water | water | 18.0 | 534 | Chemie (HOH) |
Sequence modifications
A: 281 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Met 280 | - | initiating methionine |
| Leu 707 | Ile 707 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 71016.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1253.6 | |
| All* | Total formula weight | 72269.9 |
