4N57

Crystal structure of aminoglycoside phosphotransferase APH(2'')-IVa ADP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	APH(2'')-Id	polymer	321	37595.9	2	UniProt (O68183)	Enterococcus casseliflavus
2	C, D, F, G (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	E, H (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	I, J (A, B)	water	water		18.0	240	Chemie (HOH)

Sequence modifications

A, B: 1 - 301 (UniProt: O68183)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 26	Glu 26	engineered mutation
Lys 30	Glu 30	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75191.7
Non-Polymers*	Number of molecules	6
	Total formula weight	951.6
All*	Total formula weight	76143.3

*Water molecules are not included.