4N3D

Crystal structure of the dimeric variant EGFP-K162Q in P61 space group

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	244	27448.8	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	C, D (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	E, F, G (A)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
4	H, I, J (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	K, L (A, B)	water	water		18.0	549	Chemie (HOH)

Sequence modifications

A, B: -11 - 232 (UniProt: P42212)

PDB	External Database	Details
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 1	-	expression tag
Cro 65	Ser 65	chromophore
Cro 65	Tyr 66	chromophore
Cro 65	Gly 67	chromophore
Gln 162	Lys 162	engineered mutation
Leu 231	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54897.6
Non-Polymers*	Number of molecules	8
	Total formula weight	583.5
All*	Total formula weight	55481.1

*Water molecules are not included.