4N0T

Core structure of the U6 small nuclear ribonucleoprotein at 1.7 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	U4/U6 snRNA-associated-splicing factor PRP24	polymer	374	43246.8	1	UniProt (P49960) Pfam (PF00076) Pfam (PF16842)	Saccharomyces cerevisiae (Baker's yeast)	U4/U6 snRNP protein
2	B (B)	U6 snRNA	polymer	72	23184.9	1	GenBank (BK006945.2)
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D, E (A, B)	water	water		18.0	696	Chemie (HOH)

Sequence modifications

A: 34 - 400 (UniProt: P49960)

PDB	External Database	Details
Met 33	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag

B: 30 - 101 (GenBank: BK006945.2)

PDB	External Database	Details
G 62	A 366296	engineered mutation
C 100	U 366334	engineered mutation
C 101	U 366335	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66431.6
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	66527.7

*Water molecules are not included.