4MVI

Crystal structure of an engineered lipocalin (Anticalin US7) in complex with the Alzheimer amyloid peptide Abeta(1-40)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Neutrophil gelatinase-associated lipocalin	polymer	188	21533.4	1	UniProt (P80188) Pfam (PF00061)	Homo sapiens (human)	NGAL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25
2	B (B)	Beta-amyloid protein 40	polymer	40	4335.9	1	UniProt (P05067) Pfam (PF03494)	Homo sapiens	Beta-APP40
3	C, D (A, B)	water	water		18.0	81	Chemie (HOH)

Sequence modifications

A: 1 - 178 (UniProt: P80188)

PDB	External Database	Details
His 28	Gln 48	engineered mutation
Val 36	Leu 56	engineered mutation
Lys 40	Ala 60	engineered mutation
Ser 41	Ile 61	engineered mutation
Trp 49	Gln 69	engineered mutation
Gly 70	Leu 90	engineered mutation
Gly 72	Arg 92	engineered mutation
Thr 73	Lys 93	engineered mutation
His 77	Asp 97	engineered mutation
Lys 79	Trp 99	engineered mutation
Ser 87	Cys 107	engineered mutation
Arg 96	Asn 116	engineered mutation
Arg 100	Tyr 120	engineered mutation
Arg 103	Leu 123	engineered mutation
Ala 106	Tyr 126	engineered mutation
Val 125	Lys 145	engineered mutation
Gln 127	Ser 147	engineered mutation
Ser 132	Tyr 152	engineered mutation
Asn 134	Lys 154	engineered mutation
Ser 179	-	expression tag
Ala 180	-	expression tag
Trp 181	-	expression tag
Ser 182	-	expression tag
His 183	-	expression tag
Pro 184	-	expression tag
Gln 185	-	expression tag
Phe 186	-	expression tag
Glu 187	-	expression tag
Lys 188	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25869.3
All*	Total formula weight	25869.3

*Water molecules are not included.