4MPP

Crystal Structure of Benzoylformate Decarboxylase Mutant H281Y/T377P/F397T/A460I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Benzoylformate decarboxylase	polymer	536	57493.0	1	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) In PDB	Pseudomonas putida	BFD, BFDC
2	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4	A	2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL TRIHYDROGEN DIPHOSPHATE	non-polymer		440.3	1	Chemie (TZD)
5	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	569	Chemie (HOH)

Sequence modifications

A: 1 - 528 (UniProt: P20906)

PDB	External Database	Details
Tyr 281	His 281	engineered mutation
Pro 377	Thr 377	engineered mutation
Thr 397	Phe 397	engineered mutation
Ile 460	Ala 460	engineered mutation
Arg 529	-	expression tag
Ser 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	57493.0
Non-Polymers*	Number of molecules	9
	Total formula weight	878.2
All*	Total formula weight	58371.1

*Water molecules are not included.