4MPP
Crystal Structure of Benzoylformate Decarboxylase Mutant H281Y/T377P/F397T/A460I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Benzoylformate decarboxylase | polymer | 536 | 57493.0 | 1 | UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | Pseudomonas putida | BFD, BFDC |
| 2 | B, C (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | D, E (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | F (A) | 2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL TRIHYDROGEN DIPHOSPHATE | non-polymer | 440.3 | 1 | Chemie (TZD) | |||
| 5 | G, H, I (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | J (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | K (A) | water | water | 18.0 | 569 | Chemie (HOH) |
Sequence modifications
A: 1 - 528 (UniProt: P20906)
| PDB | External Database | Details |
|---|---|---|
| Tyr 281 | His 281 | engineered mutation |
| Pro 377 | Thr 377 | engineered mutation |
| Thr 397 | Phe 397 | engineered mutation |
| Ile 460 | Ala 460 | engineered mutation |
| Arg 529 | - | expression tag |
| Ser 530 | - | expression tag |
| His 531 | - | expression tag |
| His 532 | - | expression tag |
| His 533 | - | expression tag |
| His 534 | - | expression tag |
| His 535 | - | expression tag |
| His 536 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 57493.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 878.2 | |
| All* | Total formula weight | 58371.1 |
