4MPJ
Phosphorylation of an active site threonine in the benzyolformate decarboxylase mutant S26T by phosphonate inactivation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Benzoylformate decarboxylase | polymer | 536 | 57569.9 | 1 | UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | Pseudomonas putida | BFD, BFDC |
| 2 | B, D (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | C (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | E (A) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 1 | Chemie (TPP) | |||
| 5 | F, G, H (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | I (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | J (A) | water | water | 18.0 | 559 | Chemie (HOH) |
Sequence modifications
A: 1 - 528 (UniProt: P20906)
| PDB | External Database | Details |
|---|---|---|
| Tpo 26 | Ser 26 | engineered mutation |
| Arg 529 | - | expression tag |
| Ser 530 | - | expression tag |
| His 531 | - | expression tag |
| His 532 | - | expression tag |
| His 533 | - | expression tag |
| His 534 | - | expression tag |
| His 535 | - | expression tag |
| His 536 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 57569.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 841.5 | |
| All* | Total formula weight | 58411.4 |
