4MPJ

Phosphorylation of an active site threonine in the benzyolformate decarboxylase mutant S26T by phosphonate inactivation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Benzoylformate decarboxylase	polymer	536	57569.9	1	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) In PDB	Pseudomonas putida	BFD, BFDC
2	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	A	THIAMINE DIPHOSPHATE	non-polymer		425.3	1	Chemie (TPP)
5	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	559	Chemie (HOH)

Sequence modifications

A: 1 - 528 (UniProt: P20906)

PDB	External Database	Details
Tpo 26	Ser 26	ENGINEERED MUTATION
Arg 529	-	EXPRESSION TAG
Ser 530	-	EXPRESSION TAG
His 531	-	EXPRESSION TAG
His 532	-	EXPRESSION TAG
His 533	-	EXPRESSION TAG
His 534	-	EXPRESSION TAG
His 535	-	EXPRESSION TAG
His 536	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	57569.9
Non-Polymers*	Number of molecules	8
	Total formula weight	841.5
All*	Total formula weight	58411.4

*Water molecules are not included.