4MOJ
Pyranose 2-oxidase H450G/V546C double mutant with 2-fluorinated glucose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Pyranose 2-oxidase | polymer | 633 | 70477.0 | 4 | UniProt (Q7ZA32) Pfam (PF05199) In PDB | Trametes ochracea (White-rot fungus) | Pyranose oxidase |
2 | A, B, C, D | DIHYDROFLAVINE-ADENINE DINUCLEOTIDE | non-polymer | 787.6 | 4 | Chemie (FDA) | |||
3 | A, B, C, D | 2-deoxy-2-fluoro-alpha-D-glucopyranose | non-polymer | 182.1 | 4 | Chemie (G2F) | |||
4 | A, B, C, D | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 4 | Chemie (1PE) | |||
5 | A, B, D | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 4 | Chemie (MES) | |||
6 | water | water | 18.0 | 1926 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 622 (UniProt: Q7ZA32)
PDB | External Database | Details |
---|---|---|
Ala 2 | Ser 2 | cloning artifact |
Gly 450 | His 450 | engineered mutation |
Cys 546 | Val 546 | engineered mutation |
Ala 623 | - | expression tag |
Ala 624 | - | expression tag |
Ala 625 | - | expression tag |
Leu 626 | - | expression tag |
Glu 627 | - | expression tag |
His 628 | - | expression tag |
His 629 | - | expression tag |
His 630 | - | expression tag |
His 631 | - | expression tag |
His 632 | - | expression tag |
His 633 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 281907.9 | |
Non-Polymers* | Number of molecules | 16 |
Total formula weight | 5612.9 | |
All* | Total formula weight | 287520.8 |