4MOI

Pyranose 2-oxidase H450G/V546C double mutant with 3-fluorinated glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pyranose 2-oxidase	polymer	633	70477.0	2	UniProt (Q7ZA32) Pfam (PF05199)	Trametes ochracea (White-rot fungus)	Pyranose oxidase
2	C, E (A, B)	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	2	Chemie (FDA)
3	D, F (A, B)	3-deoxy-3-fluoro-beta-D-glucopyranose	non-polymer		182.1	2	Chemie (G3F)
4	G, H (A, B)	water	water		18.0	635	Chemie (HOH)

Sequence modifications

A, B: 1 - 622 (UniProt: Q7ZA32)

PDB	External Database	Details
Ala 2	Ser 2	SEE REMARK 999
Gly 450	His 450	engineered mutation
Cys 546	Val 546	engineered mutation
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag
Leu 626	-	expression tag
Glu 627	-	expression tag
His 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	140953.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1939.4
All*	Total formula weight	142893.4

*Water molecules are not included.