4MMQ

Crystal Structure of Prefusion-stabilized RSV F Variant DS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fusion glycoprotein F2	polymer	82	9215.4	1	UniProt (P03420)	Human respiratory syncytial virus A2
2	B (B)	Fusion glycoprotein F1 fused with Fibritin trimerization domain	polymer	414	45535.0	1	UniProt (P03420) UniProt (P10104) Pfam (PF00523)	Human respiratory syncytial virus A2
3	C, D, E, F, G... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)

Sequence modifications

A: 26 - 107 (UniProt: P03420)

PDB	External Database	Details
Ala 102	Pro 102	engineered mutation

B: 137 - 513 (UniProt: P03420)

PDB	External Database	Details
Cys 155	Ser 155	engineered mutation
Cys 290	Ser 290	engineered mutation
Val 379	Ile 379	engineered mutation
Val 447	Met 447	engineered mutation
Ser 514	-	linker
Ala 515	-	linker
Ile 516	-	linker
Gly 517	-	linker

B: 518 - 544 (UniProt: P10104)

PDB	External Database	Details
Leu 539	Phe 479	variant
Gly 545	-	expression tag
Gly 546	-	expression tag
Leu 547	-	expression tag
Val 548	-	expression tag
Pro 549	-	expression tag
Arg 550	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54750.4
Non-Polymers*	Number of molecules	6
	Total formula weight	576.4
All*	Total formula weight	55326.8

*Water molecules are not included.