4MMI

Crystal structure of heparan sulfate lyase HepC mutant from Pedobacter heparinus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Heparinase III protein	polymer	643	74341.5	1	UniProt (Q59289) Pfam (PF16889) Pfam (PF07940)	Pedobacter heparinus
2	B, C (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	D (A)	water	water		18.0	61	Chemie (HOH)

Sequence modifications

A: 25 - 659 (UniProt: Q59289)

PDB	External Database	Details
Val 29	Ile 29	engineered mutation
Ser 657	Leu 657	engineered mutation
Leu 660	-	expression tag
Glu 661	-	expression tag
His 662	-	expression tag
His 663	-	expression tag
His 664	-	expression tag
His 665	-	expression tag
His 666	-	expression tag
His 667	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74341.5
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	74421.6

*Water molecules are not included.