4MKH
Crystal structure of a stable adenylate kinase variant AKv18
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Adenylate kinase | polymer | 222 | 24967.6 | 1 | UniProt (P16304) Pfam (PF00406) Pfam (PF05191) | Bacillus subtilis | AK, ATP-AMP transphosphorylase, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Superoxide-inducible protein 16, SOI16 |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C (A) | BIS(ADENOSINE)-5'-PENTAPHOSPHATE | non-polymer | 916.4 | 1 | Chemie (AP5) | |||
| 4 | D, E, F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 5 | G (A) | water | water | 18.0 | 135 | Chemie (HOH) |
Sequence modifications
A: 1 - 212 (UniProt: P16304)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ile 3 | Leu 3 | engineered mutation |
| Leu 16 | Gln 16 | engineered mutation |
| Ala 17 | Gly 17 | engineered mutation |
| Lys 19 | Arg 19 | engineered mutation |
| Lys 23 | Asp 23 | engineered mutation |
| Arg 69 | Lys 69 | engineered mutation |
| Ser 73 | Gly 73 | engineered mutation |
| Ser 75 | Asp 75 | engineered mutation |
| Met 103 | Tyr 103 | engineered mutation |
| Arg 105 | Lys 105 | engineered mutation |
| Gln 114 | Glu 114 | engineered mutation |
| Arg 116 | Asp 116 | engineered mutation |
| Glu 118 | Asp 118 | engineered mutation |
| Glu 119 | Val 119 | engineered mutation |
| Thr 169 | Ser 169 | engineered mutation |
| Met 179 | Thr 179 | engineered mutation |
| Ala 180 | Gln 180 | engineered mutation |
| Ala 184 | Asp 184 | engineered mutation |
| Asp 187 | Ser 187 | engineered mutation |
| Ser 188 | Glu 188 | engineered mutation |
| Glu 190 | Gly 190 | engineered mutation |
| Val 191 | Tyr 191 | engineered mutation |
| Val 193 | Ala 193 | engineered mutation |
| Glu 198 | Gln 198 | engineered mutation |
| Arg 199 | Gln 199 | engineered mutation |
| Glu 202 | Gln 202 | engineered mutation |
| Phe 205 | Tyr 205 | engineered mutation |
| Asp 209 | Lys 209 | engineered mutation |
| Val 210 | Asp 210 | engineered mutation |
| Ile 211 | Leu 211 | engineered mutation |
| Leu 213 | - | expression tag |
| Glu 214 | - | expression tag |
| His 215 | - | expression tag |
| His 216 | - | expression tag |
| His 217 | - | expression tag |
| His 218 | - | expression tag |
| His 219 | - | expression tag |
| His 220 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 24967.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1168.0 | |
| All* | Total formula weight | 26135.6 |
