4MFQ

The crystal structure of acyltransferase in complex with CoA and 10C-Teicoplanin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative uncharacterized protein tcp24	polymer	345	39204.3	1	UniProt (Q70AY4) Pfam (PF18082) Pfam (PF18164)	Actinoplanes teichomyceticus	acyltransferase
2	B (B)	Teicoplanin pseudoaglycone	polymer	7	1207.0	1	BIRD (PRD_001169)	Actinoplanes teichomyceticus
3	C (A)	COENZYME A	non-polymer		767.5	1	Chemie (COA)
4	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	F (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	BIRD (PRD_001169) Chemie (NAG)
6	G (B)	alpha-D-mannopyranose	non-polymer		180.2	1	BIRD (PRD_001169) Chemie (MAN)
7	H (B)	2-amino-2-deoxy-beta-D-glucopyranose	non-polymer		179.2	1	BIRD (PRD_001169) Chemie (GCS)
8	I (B)	DECANOIC ACID	non-polymer		172.3	1	BIRD (PRD_001169) Chemie (DKA)
9	J (B)	D-ALANINE	non-polymer		89.1	1	Chemie (DAL)
10	K, L (A, B)	water	water		18.0	311	Chemie (HOH)

Sequence modifications

A: 1 - 323 (UniProt: Q70AY4)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Met 0	-	expression tag
Ala 196	His 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40411.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1801.6
All*	Total formula weight	42212.9

*Water molecules are not included.