4MB5
Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Chitinase 60 | polymer | 528 | 58616.4 | 1 | UniProt (B1VBB0) Pfam (PF00704) Pfam (PF16403) Pfam (PF02839) | Moritella marina | |
| 2 | B (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose | branched | 1034.0 | 1 | In PDB GlyTouCan (G46227HN) | |||
| 3 | C (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | D, E, I (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | F, K (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 6 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | H, J (A) | GLYCINE | non-polymer | 75.1 | 2 | Chemie (GLY) | |||
| 8 | L (A) | water | water | 18.0 | 700 | Chemie (HOH) |
Sequence modifications
A: 23 - 550 (UniProt: B1VBB0)
| PDB | External Database | Details |
|---|---|---|
| Gln 153 | Glu 153 | engineered mutation |
| His 452 | Arg 452 | SEE REMARK 999 |
| Thr 470 | Ala 470 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 58616.4 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1034.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 757.7 | |
| All* | Total formula weight | 60408.1 |
