4M81
The structure of E292S glycosynthase variant of exo-1,3-beta-glucanase from Candida albicans complexed with 1-fluoro-alpha-D-glucopyranoside (donor) and p-nitrophenyl beta-D-glucopyranoside (acceptor) at 1.86A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | EXO-1,3-BETA-GLUCANASE | polymer | 399 | 45693.3 | 1 | UniProt (Q5AI63) Pfam (PF00150) UniProt (by SIFTS) (P29717) | Candida albicans (yeast) | |
| 2 | B (A) | alpha-D-glucopyranosyl fluoride | non-polymer | 182.1 | 1 | Chemie (GLF) | |||
| 3 | C (A) | 4-nitrophenyl beta-D-glucopyranoside | non-polymer | 301.2 | 1 | Chemie (PNW) | |||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | E (A) | water | water | 18.0 | 492 | Chemie (HOH) |
Sequence modifications
A: 2 - 400 (UniProt: Q5AI63)
| PDB | External Database | Details |
|---|---|---|
| Leu 64 | Ser 102 | SEE REMARK 999 |
| Ser 292 | Glu 330 | engineered mutation |
| Leu 329 | Met 367 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 45693.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 575.5 | |
| All* | Total formula weight | 46268.8 |
