4M81
The structure of E292S glycosynthase variant of exo-1,3-beta-glucanase from Candida albicans complexed with 1-fluoro-alpha-D-glucopyranoside (donor) and p-nitrophenyl beta-D-glucopyranoside (acceptor) at 1.86A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | EXO-1,3-BETA-GLUCANASE | polymer | 399 | 45693.3 | 1 | UniProt (Q5AI63) Pfam (PF00150) UniProt (by SIFTS) (P29717) | Candida albicans (yeast) | |
| 2 | B (A) | alpha-D-glucopyranosyl fluoride | non-polymer | 182.1 | 1 | Chemie (GLF) PubChem (21600205) PubChem (150967) PubChem (444915) PubChem (4465565) PubChem (10921145) PubChem (11126887) PubChem (11286835) PubChem (11412739) PubChem (11816235) PubChem (13643247) PubChem (129668793) PubChem (129685662) PubChem (131107081) PubChem (131215288) PubChem (133620782) PubChem (135085714) PubChem (157225707) PubChem (157225708) PubChem (163321034) PubChem (166604477) PubChem (172641965) | |||
| 3 | C (A) | 4-nitrophenyl beta-D-glucopyranoside | non-polymer | 301.2 | 1 | Chemie (PNW) | |||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 5 | E (A) | water | water | 18.0 | 492 | Chemie (HOH) |
Sequence modifications
A: 2 - 400 (UniProt: Q5AI63)
| PDB | External Database | Details |
|---|---|---|
| Leu 64 | Ser 102 | SEE REMARK 999 |
| Ser 292 | Glu 330 | engineered mutation |
| Leu 329 | Met 367 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 45693.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 575.5 | |
| All* | Total formula weight | 46268.8 |
