4M75

Crystal structure of Lsm1-7 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, H (A, H)	U6 snRNA-associated Sm-like protein Lsm1	polymer	144	17473.8	2	UniProt (P47017) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
2	B, I (B, I)	U6 snRNA-associated Sm-like protein Lsm2	polymer	95	11302.5	2	UniProt (P38203) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
3	C, J (C, J)	U6 snRNA-associated Sm-like protein Lsm3	polymer	89	10100.9	2	UniProt (P57743) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
4	D, K (D, K)	U6 snRNA-associated Sm-like protein Lsm6	polymer	86	9547.3	2	UniProt (Q06406) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
5	E, L (E, L)	U6 snRNA-associated Sm-like protein Lsm5	polymer	93	10573.6	2	UniProt (P40089) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
6	F, M (F, M)	U6 snRNA-associated Sm-like protein Lsm7	polymer	115	13261.5	2	UniProt (P53905) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
7	G, N (G, N)	U6 snRNA-associated Sm-like protein Lsm4	polymer	122	14088.3	2	UniProt (P40070) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)
8	O, P (B, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)

Sequence modifications

A, H: 30 - 172 (UniProt: P47017)

PDB	External Database	Details
Mse 29	-	expression tag

B, I: 1 - 95 (UniProt: P38203)

PDB	External Database	Details
Ser 45	Cys 45	engineered mutation

C, J: 1 - 89 (UniProt: P57743)

PDB	External Database	Details
Ser 37	Cys 37	engineered mutation
Ser 63	Cys 63	engineered mutation

G, N: 1 - 93 (UniProt: P40070)

PDB	External Database	Details
Mse -28	-	expression tag
Lys -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Ala -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	172696.0
Non-Polymers*	Number of molecules	2
	Total formula weight	70.9
All*	Total formula weight	172766.9

*Water molecules are not included.