4M72

Mutant structure of methyltransferase from Streptomyces hygroscopicus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methyltransferase MppJ	polymer	337	37214.9	2	UniProt (Q643C8)	Streptomyces hygroscopicus
2	C, L (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (71170554) PubChem (13231890) PubChem (16760024) PubChem (25210495) PubChem (44361184) PubChem (177678424) PubChem (46936206) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (6419189) PubChem (57544532) PubChem (59046843) PubChem (59170628) PubChem (164157219) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
3	D, M (A, B)	(2R,3R)-2-hydroxy-3-methoxy-3-phenylpropanoic acid	non-polymer		196.2	2	Chemie (M72) PubChem (74766017)
4	E, N (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE) PubChem (29936)
5	F, O (A, B)	(2R)-2-hydroxy-3-phenylpropanoic acid	non-polymer		166.2	2	Chemie (HF2)
6	G, H, I, J, K... (A, B)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA) PubChem (271)
7	S, T (A, B)	water	water		18.0	759	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: Q643C8)

PDB	External Database	Details
Leu 127	Arg 127	engineered mutation
Glu 300	Val 300	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74429.8
Non-Polymers*	Number of molecules	16
	Total formula weight	1925.9
All*	Total formula weight	76355.6

*Water molecules are not included.