4M72
Mutant structure of methyltransferase from Streptomyces hygroscopicus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Methyltransferase MppJ | polymer | 337 | 37214.9 | 2 | UniProt (Q643C8) Pfam (PF13649) | Streptomyces hygroscopicus | |
| 2 | C, L (A, B) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) | |||
| 3 | D, M (A, B) | (2R,3R)-2-hydroxy-3-methoxy-3-phenylpropanoic acid | non-polymer | 196.2 | 2 | Chemie (M72) | |||
| 4 | E, N (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
| 5 | F, O (A, B) | (2R)-2-hydroxy-3-phenylpropanoic acid | non-polymer | 166.2 | 2 | Chemie (HF2) | |||
| 6 | G, H, I, J, K... (A, B) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 7 | S, T (A, B) | water | water | 18.0 | 759 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q643C8)
| PDB | External Database | Details |
|---|---|---|
| Leu 127 | Arg 127 | engineered mutation |
| Glu 300 | Val 300 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74429.8 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1925.9 | |
| All* | Total formula weight | 76355.6 |
