4M6Y
Mutant structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-homocysteine and methylphenylpyruvic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Methyltransferase MppJ | polymer | 357 | 39398.4 | 2 | UniProt (Q643C8) | Streptomyces hygroscopicus | |
| 2 | C, K (A, B) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (71170554) PubChem (13231890) PubChem (16760024) PubChem (25210495) PubChem (44361184) PubChem (177678424) PubChem (46936206) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (6419189) PubChem (57544532) PubChem (59046843) PubChem (59170628) PubChem (164157219) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 3 | D, L (A, B) | (3R)-2-oxo-3-phenylbutanoic acid | non-polymer | 178.2 | 2 | Chemie (56D) PubChem (13065771) PubChem (70243965) PubChem (73659125) | |||
| 4 | E, M (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) PubChem (29936) | |||
| 5 | F, N (A, B) | (2R)-2-hydroxy-3-phenylpropanoic acid | non-polymer | 166.2 | 2 | Chemie (HF2) | |||
| 6 | G, H, I, O (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 7 | J, P (A, B) | OXYGEN MOLECULE | non-polymer | 32.0 | 2 | Chemie (OXY) PubChem (977) | |||
| 8 | Q, R (A, B) | water | water | 18.0 | 328 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q643C8)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Leu 244 | Asp 244 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 78796.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1793.5 | |
| All* | Total formula weight | 80590.3 |
