4M4N

Structural evaluation the Y358H mutant of the aspergillus fumigatus kdnase (sialidase)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Extracellular sialidase/neuraminidase, putative	polymer	422	46251.6	2	UniProt (Q4WQS0) Pfam (PF13088)	Aspergillus fumigatus
2	C, G (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	D, E, F, H, I (A, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	J, K (A, B)	water	water		18.0	1177	Chemie (HOH)

Sequence modifications

A, B: 1 - 406 (UniProt: Q4WQS0)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 358	Tyr 358	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92503.1
Non-Polymers*	Number of molecules	7
	Total formula weight	506.4
All*	Total formula weight	93009.6

*Water molecules are not included.