4M35

Crystal structure of gated-pore mutant H126/141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative starvation-induced DNA protecting protein/Ferritin and Dps	polymer	168	18466.6	4	UniProt (A0QXB7) Pfam (PF00210) In PDB	Mycobacterium smegmatis	Starvation-inducible DNA-binding protein or fine tangled pili major subunit
2	A, B, C, D	FE (II) ION	non-polymer		55.8	4	Chemie (FE2)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, D	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5		water	water		18.0	384	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 161 (UniProt: A0QXB7)

PDB	External Database	Details
Met -6	-	expression tag
Ala -5	-	expression tag
Ser -4	-	expression tag
Val -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag
Asp 126	His 126	engineered mutation
Asp 141	His 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	73866.3
Non-Polymers*	Number of molecules	8
	Total formula weight	342.9
All*	Total formula weight	74209.2

*Water molecules are not included.