4M04

Human DNA Polymerase Mu ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed DNA/RNA polymerase mu	polymer	356	40054.4	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (human)	Pol Mu, Terminal transferase
2	T	template strand	polymer	9	2740.8	1
3	P	upstream primer strand	polymer	4	1190.8	1
4	D	downstream primer strand	polymer	4	1191.8	1
5	A	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
6	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
10	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
11		water	water		18.0	381	Chemie (HOH)

Sequence modifications

A: 132 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 127	-	expression tag
Ser 128	-	expression tag
Ala 129	-	expression tag
Ala 130	-	expression tag
Ala 131	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
-	Pro 410	deletion
Gly 410	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45177.9
Non-Polymers*	Number of molecules	9
	Total formula weight	998.7
All*	Total formula weight	46176.6

*Water molecules are not included.