4LRT
Crystal and solution structures of the bifunctional enzyme (Aldolase/Aldehyde dehydrogenase) from Thermomonospora curvata, reveal a cofactor-binding domain motion during NAD+ and CoA accommodation whithin the shared cofactor-binding site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | 4-hydroxy-2-oxovalerate aldolase | polymer | 355 | 37326.9 | 2 | UniProt (D1A3K8) Pfam (PF00682) Pfam (PF07836) In PDB | Thermomonospora curvata | HOA, 4-hydroxy-2-keto-pentanoic acid aldolase, 4-hydroxy-2-oxopentanoate aldolase |
2 | B, D | Acetaldehyde dehydrogenase | polymer | 323 | 34127.8 | 2 | UniProt (D1A3K7) Pfam (PF01118) Pfam (PF09290) In PDB | Thermomonospora curvata | Acetaldehyde dehydrogenase [acetylating] |
3 | A, C | PYRUVIC ACID | non-polymer | 88.1 | 2 | Chemie (PYR) | |||
4 | A, B, D | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
5 | A, C | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | B, D | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
7 | B, C | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
8 | B, D | COENZYME A | non-polymer | 767.5 | 2 | Chemie (COA) | |||
9 | water | water | 18.0 | 1364 | Chemie (HOH) |
Sequence modifications
B, D: 21 - 323 (UniProt: D1A3K7)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
Ser 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
Ser 11 | - | expression tag |
Ser 12 | - | expression tag |
Gly 13 | - | expression tag |
Leu 14 | - | expression tag |
Val 15 | - | expression tag |
Pro 16 | - | expression tag |
Arg 17 | - | expression tag |
Gly 18 | - | expression tag |
Ser 19 | - | expression tag |
His 20 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 142909.4 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 2320.2 | |
All* | Total formula weight | 145229.5 |