4LQT

1.10A resolution crystal structure of a superfolder green fluorescent protein (W57A) mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	248	27698.1	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	B, C (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
3	D (A)	water	water		18.0	257	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ala 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Ala 57	Trp 57	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27698.1
Non-Polymers*	Number of molecules	2
	Total formula weight	124.1
All*	Total formula weight	27822.2

*Water molecules are not included.