4LCD

Structure of an Rsp5xUbxSna3 complex: Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	E3 ubiquitin-protein ligase RSP5	polymer	432	50470.7	2	UniProt (P39940) Pfam (PF00397) Pfam (PF00632) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Reverses SPT-phenotype protein 5
2	C, D	Protein SNA3	polymer	24	2560.6	2	UniProt (P14359) In PDB	Saccharomyces cerevisiae
3	E, F	Ubiquitin	polymer	83	9583.0	2	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)

Sequence modifications

A, B: 383 - 809 (UniProt: P39940)

PDB	External Database	Details
Gly 378	-	expression tag
Ser 379	-	expression tag
Gly 380	-	expression tag
Gly 381	-	expression tag
Ser 382	-	expression tag
Leu 455	Cys 455	engineered mutation
Gly 517	Cys 517	engineered mutation
Ala 721	Cys 721	engineered mutation

C, D: 104 - 127 (UniProt: P14359)

PDB	External Database	Details
Ala 125	Lys 125	engineered mutation

E, F: 1 - 75 (UniProt: P0CG48)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cys 75	Gly 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	125228.6
All*	Total formula weight	125228.6

*Water molecules are not included.