4L64

Crystal structure of the Candida albicans Methionine Synthase in complex with 5-Methyl-Tetrahydrofolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase	polymer	789	88189.0	1	UniProt (P82610) Pfam (PF08267) Pfam (PF01717) In PDB	Candida albicans SC5314	Cobalamin-independent methionine synthase, Methionine synthase, vitamin-B12 independent isozyme
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	5-METHYL-5,6,7,8-TETRAHYDROFOLIC ACID	non-polymer		459.5	1	Chemie (C2F)
4		water	water		18.0	312	Chemie (HOH)

Sequence modifications

A: 1 - 767 (UniProt: P82610)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Ala 103	Lys 103	engineered mutation
Ala 104	Lys 104	engineered mutation
Ala 107	Glu 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	88189.0
Non-Polymers*	Number of molecules	2
	Total formula weight	524.9
All*	Total formula weight	88713.8

*Water molecules are not included.