4L27

Crystal structure of delta1-39 and delta516-525 human cystathionine beta-synthase D444N mutant containing C-terminal 6xHis tag

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, D, A, C)	Cystathionine beta-synthase	polymer	548	60513.2	4	UniProt (P35520) Pfam (PF00291) Pfam (PF00571)	Homo sapiens (human)	Beta-thionase, Serine sulfhydrase
2	E, G, I, K (B, D, A, C)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	4	Chemie (PLP)
3	F, H, J, L (B, D, A, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)

Sequence modifications

B, D, A, C: 2 - 551 (UniProt: P35520)

PDB	External Database	Details
Asn 444	Asp 444	engineered mutation
-	Ile 516	deletion
-	Gln 517	deletion
-	Tyr 518	deletion
-	His 519	deletion
-	Ser 520	deletion
-	Thr 521	deletion
-	Gly 522	deletion
-	Lys 523	deletion
-	Ser 524	deletion
-	Ser 525	deletion
Leu 552	-	expression tag
Glu 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	242052.8
Non-Polymers*	Number of molecules	8
	Total formula weight	3454.5
All*	Total formula weight	245507.3

*Water molecules are not included.