4L1Q

Crystal Structure of the E113Q-MauG/pre-Methylamine Dehydrogenase Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methylamine utilization protein MauG	polymer	373	41145.6	2	UniProt (Q51658) Pfam (PF03150)	Paracoccus denitrificans
2	C, E (C, E)	Methylamine dehydrogenase light chain	polymer	137	15025.6	2	UniProt (A1BBA0) Pfam (PF02975)	Paracoccus denitrificans
3	D, F (D, F)	Methylamine dehydrogenase heavy chain	polymer	385	42321.2	2	UniProt (A1BB97) Pfam (PF06433)	Paracoccus denitrificans
4	G, L (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	H, I, M, N (A, B)	HEME C	non-polymer		618.5	4	Chemie (HEC)
6	J, O, P (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
7	K, Q, R (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
8	S, T (D, F)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
9	U, V, W, X, Y... (A, B, C, D, E...)	water	water		18.0	1521	Chemie (HOH)

Sequence modifications

A, B: 1 - 367 (UniProt: Q51658)

PDB	External Database	Details
Gln 113	Glu 133	engineered mutation
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

C, E: 1 - 131 (UniProt: A1BBA0)

PDB	External Database	Details
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	196984.8
Non-Polymers*	Number of molecules	14
	Total formula weight	3199.8
All*	Total formula weight	200184.6

*Water molecules are not included.