4KYS
Clostridium botulinum thiaminase I in complex with thiamin
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Thiamine pyridinylase I | polymer | 427 | 49009.8 | 2 | UniProt (A7FS27) In PDB | Clostridium botulinum | Cb-thiaminase I |
2 | A, B | 3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM | non-polymer | 265.4 | 2 | Chemie (VIB) | |||
3 | A, B | CITRIC ACID | non-polymer | 192.1 | 2 | Chemie (CIT) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
5 | water | water | 18.0 | 430 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 404 (UniProt: A7FS27)
PDB | External Database | Details |
---|---|---|
Met -22 | - | expression tag |
Gly -21 | - | expression tag |
Ser -20 | - | expression tag |
Asp -19 | - | expression tag |
Lys -18 | - | expression tag |
Ile -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
Gly -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
Ser 143 | Cys 143 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 98019.6 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 1012.2 | |
All* | Total formula weight | 99031.8 |