4KYI

Crystal structure of the phospholipase VipD from Legionella pneumophila in complex with the human GTPase Rab5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	VipD	polymer	551	61229.7	4	UniProt (Q5ZRP9) Pfam (PF01734) Pfam (PF22140)	Legionella pneumophila subsp. pneumophila
2	B, D, F, H (B, D, F, H)	Ras-related protein Rab-5C	polymer	170	18791.5	4	UniProt (P51148) Pfam (PF00071)	Homo sapiens (human)	L1880, RAB5L
3	I, J, N, O, S... (A, B, E, G)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4	K, L, T (A, E)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	M, Q, U, X (B, D, F, H)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	4	Chemie (GNP)
6	P, R, V, Y (B, D, F, H)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, C, E, G: 19 - 564 (UniProt: Q5ZRP9)

PDB	External Database	Details
Gly 14	-	expression tag
Ala 15	-	expression tag
Met 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag

B, D, F, H: 18 - 182 (UniProt: P51148)

PDB	External Database	Details
Gly 13	-	expression tag
Ala 14	-	expression tag
Met 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Leu 80	Gln 80	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	320084.7
Non-Polymers*	Number of molecules	17
	Total formula weight	2834.7
All*	Total formula weight	322919.4

*Water molecules are not included.