4KVL

Crystal structure of Oryza sativa fatty acid alpha-dioxygenase Y379F with palmitic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Fatty acid alpha-oxidase	polymer	621	70951.6	1	UniProt (Q9M5J1) Pfam (PF03098) UniProt (by SIFTS) (Q2QRV3) In PDB	Oryza sativa (red rice)
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
4	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	A	octyl beta-D-glucopyranoside	non-polymer		292.4	2	Chemie (BOG)
7	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
8	A	TRIETHYLENE GLYCOL	non-polymer		150.2	6	Chemie (PGE)
9	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
10	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
11		water	water		18.0	505	Chemie (HOH)

Sequence modifications

A: 10 - 618 (UniProt: Q9M5J1)

PDB	External Database	Details
Met -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Phe 379	Tyr 379	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70951.6
Non-Polymers*	Number of molecules	17
	Total formula weight	3227.7
All*	Total formula weight	74179.3

*Water molecules are not included.