4KRV

Crystal structure of catalytic domain of bovine beta1,4-galactosyltransferase mutant M344H-GalT1 complex with 6-sulfo-GlcNAc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-1,4-galactosyltransferase 1	polymer	286	32856.5	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (bovine cow domestic cattle domestic cow)	Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1
2	C, L (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, M (A, B)	6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE	non-polymer		503.3	2	Chemie (UDH)
4	E, N (A, B)	2-acetamido-2-deoxy-6-O-sulfo-beta-D-glucopyranose	non-polymer		301.3	2	Chemie (NGS)
5	F, G, H, I, J... (A, B)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
6	S, T (A, B)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A, B: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	expression tag
Ser 118	-	expression tag
Met 119	-	expression tag
Thr 120	-	expression tag
Gly 121	-	expression tag
Gly 122	-	expression tag
Gln 123	-	expression tag
Gln 124	-	expression tag
Met 125	-	expression tag
Gly 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
Thr 342	Cys 342	engineered mutation
His 344	Met 344	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65713.1
Non-Polymers*	Number of molecules	16
	Total formula weight	2679.7
All*	Total formula weight	68392.8

*Water molecules are not included.