4KPB

Crystal structure of cytochrome P450 BM-3 R47E mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cytochrome P450 BM-3	polymer	494	56488.1	2	UniProt (P14779) Pfam (PF00067) In PDB	Bacillus megaterium	Bifunctional P-450/NADPH-P450 reductase, Cytochrome P450(BM-3), Cytochrome P450 102
2	A, B	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3		water	water		18.0	772	Chemie (HOH)

Sequence modifications

A, B: 0 - 470 (UniProt: P14779)

PDB	External Database	Details
Met -23	-	expression tag
Gly -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Glu 47	Arg 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	112976.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1233.0
All*	Total formula weight	114209.3

*Water molecules are not included.