4KMM

M76H variant of human ferrochelatase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ferrochelatase, mitochondrial	polymer	370	42403.6	2	UniProt (P22830) Pfam (PF00762)	Homo sapiens (human)	Heme synthase, Protoheme ferro-lyase
2	C, H (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	D, I (A, B)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
4	E, F, J, K (A, B)	CHOLIC ACID	non-polymer		408.6	4	Chemie (CHD)
5	G, L (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	M, N (A, B)	water	water		18.0	109	Chemie (HOH)

Sequence modifications

A, B: 65 - 423 (UniProt: P22830)

PDB	External Database	Details
Met 54	-	expression tag
Gly 55	-	expression tag
Gly 56	-	expression tag
Ser 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
Gly 64	-	expression tag
His 76	Met 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84807.3
Non-Polymers*	Number of molecules	10
	Total formula weight	3289.8
All*	Total formula weight	88097.1

*Water molecules are not included.