4KLY

Crystal structure of a blue-light absorbing proteorhodopsin mutant D97N from HOT75

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Blue-light absorbing proteorhodopsin	polymer	259	28393.4	5	UniProt (Q9AFF7) Pfam (PF01036) In PDB	gamma proteobacterium 'Hot 75m4'	BPR
2	A, B, C, D, E	RETINAL	non-polymer		284.4	5	Chemie (RET)
3		water	water		18.0	13	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 0 - 250 (UniProt: Q9AFF7)

PDB	External Database	Details
Asn 97	Asp 98	ENGINEERED MUTATION
Leu 251	-	EXPRESSION TAG
Glu 252	-	EXPRESSION TAG
His 253	-	EXPRESSION TAG
His 254	-	EXPRESSION TAG
His 255	-	EXPRESSION TAG
His 256	-	EXPRESSION TAG
His 257	-	EXPRESSION TAG
His 258	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	141967.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1422.2
All*	Total formula weight	143389.2

*Water molecules are not included.