4KLR

E343Q variant of human ferrochelatase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ferrochelatase, mitochondrial	polymer	370	42395.7	2	UniProt (P22830) Pfam (PF00762) In PDB	Homo sapiens (human)	Heme synthase, Protoheme ferro-lyase
2	A, B	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
3	A, B	BICARBONATE ION	non-polymer		61.0	2	Chemie (BCT)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A, B	CHOLIC ACID	non-polymer		408.6	5	Chemie (CHD)
6	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	A, B	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
8	A, B	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
9		water	water		18.0	341	Chemie (HOH)

Sequence modifications

A, B: 65 - 423 (UniProt: P22830)

PDB	External Database	Details
Met 54	-	expression tag
Gly 55	-	expression tag
Gly 56	-	expression tag
Ser 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
Gly 64	-	expression tag
Gln 343	Glu 343	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84791.4
Non-Polymers*	Number of molecules	15
	Total formula weight	4015.2
All*	Total formula weight	88806.6

*Water molecules are not included.