4KL5

Crystal structure of NpuDnaE intein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA polymerase III, alpha subunit, Nucleic acid binding, OB-fold, tRNA/helicase-type chimeric construct	polymer	144	16377.4	2	UniProt (B2J066) UniProt (B2J821)	Nostoc punctiforme
2	C, D (A)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
3	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G (A, B)	water	water		18.0	221	Chemie (HOH)

Sequence modifications

A, B: 1 - 102 (UniProt: B2J066)

PDB	External Database	Details
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ala 1	Cys 775	engineered mutation

A, B: 103 - 141 (UniProt: B2J821)

PDB	External Database	Details
Ala 138	Cys 37	engineered mutation
Asp 139	Phe 38	engineered mutation
Gly 141	Lys 40	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32754.8
Non-Polymers*	Number of molecules	3
	Total formula weight	476.3
All*	Total formula weight	33231.1

*Water molecules are not included.