4KIT

Crystal structure of human Brr2 in complex with the Prp8 Jab1/MPN domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	U5 small nuclear ribonucleoprotein 200 kDa helicase	polymer	1739	198785.8	1	UniProt (O75643) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) In PDB	Homo sapiens (human)	Brr2 protein, Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein, U5-200KD
2	C	Pre-mRNA-processing-splicing factor 8	polymer	278	31861.0	1	UniProt (Q6P2Q9) Pfam (PF01398) Pfam (PF08084) In PDB	Homo sapiens (human)	Prp8 protein, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
3	B	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B: 395 - 2129 (UniProt: O75643)

PDB	External Database	Details
Gly 391	-	expression tag
Ala 392	-	expression tag
Glu 393	-	expression tag
Phe 394	-	expression tag

C: 2064 - 2335 (UniProt: Q6P2Q9)

PDB	External Database	Details
Gly 2058	-	expression tag
Pro 2059	-	expression tag
Leu 2060	-	expression tag
Gly 2061	-	expression tag
Ser 2062	-	expression tag
Met 2063	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	230646.8
Non-Polymers*	Number of molecules	3
	Total formula weight	878.7
All*	Total formula weight	231525.5

*Water molecules are not included.