4KIF

Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with phenylpyruvic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methyltransferase MppJ	polymer	357	39400.3	2	UniProt (Q643C8) Pfam (PF13649)	Streptomyces hygroscopicus
2	C, F, J (A, B)	3-PHENYLPYRUVIC ACID	non-polymer		164.2	3	Chemie (PPY)
3	D, K (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
4	E, L (A, B)	(2R)-2-hydroxy-3-phenylpropanoic acid	non-polymer		166.2	2	Chemie (HF2)
5	G, H, I, M (A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	N, O (A, B)	water	water		18.0	347	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: Q643C8)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78800.6
Non-Polymers*	Number of molecules	11
	Total formula weight	1096.8
All*	Total formula weight	79897.4

*Water molecules are not included.