4KHZ

Crystal structure of the maltose-binding protein/maltose transporter complex in an pre-translocation conformation bound to maltoheptaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	Maltose-binding periplasmic protein	polymer	380	41898.3	1	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli	MBP, MMBP, Maltodextrin-binding protein
2	B (F)	Maltose transport system permease protein MalF	polymer	514	57052.9	1	UniProt (P02916) Pfam (PF20872) Pfam (PF14785) Pfam (PF00528)	Escherichia coli
3	C (G)	Binding-protein-dependent transport systems inner membrane component	polymer	296	32246.2	1	UniProt (C9QV46)	Escherichia coli	Part of maltose permease, inner membrane component
4	D, E (A, B)	Binding-protein-dependent transport systems inner membrane component	polymer	381	42184.5	2	UniProt (C9QV42)	Escherichia coli	Part of maltose permease, inner membrane component
5	F (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		666.6	1	In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ)		alpha-maltotetraose
6	G (D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
7	H (F)	(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE	non-polymer		749.0	1	Chemie (PGV)
8	I, J, K, L, M (E, F, G, A, B)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

E: 1 - 370 (UniProt: P0AEX9)

PDB	External Database	Details
Ala 371	-	expression tag
Ser 372	-	expression tag
Ala 373	-	expression tag
Ser 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag

A, B: 1 - 371 (UniProt: C9QV42)

PDB	External Database	Details
Ala 372	-	expression tag
Ser 373	-	expression tag
Ala 374	-	expression tag
Ser 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	215566.5
Branched	Number of molecules	2
	Total formula weight	1171.0
Non-Polymers*	Number of molecules	1
	Total formula weight	749.0
All*	Total formula weight	217486.6

*Water molecules are not included.