4KHN

Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, B)	DNA polymerase	polymer	903	104519.0	2	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Enterobacteria phage RB69	Gp43
2	B, D (C, E)	DNA (5'-D(*TP*CP*AP*CP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3')	polymer	18	5501.6	2
3	C, E (D, F)	DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3')	polymer	13	3967.6	2
4	G, Q, R, S (A, C, B)	2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine	non-polymer		506.2	4	Chemie (XG4)
5	H, U (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	I, J, K, L, M... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
7	N, O (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	P (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
9	AA, V, W, X, Y... (B, A, C, D, E...)	water	water		18.0	304	Chemie (HOH)

Sequence modifications

A, B: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
Ala 567	Tyr 567	engineered mutation
Ala 714	Asp 714	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	227976.4
Non-Polymers*	Number of molecules	15
	Total formula weight	2888.5
All*	Total formula weight	230864.9

*Water molecules are not included.