4KEA

Crystal structure of D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in space group P212121

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Thermostable monoacylglycerol lipase	polymer	270	29559.6	6	UniProt (P82597) Pfam (PF12146)	Bacillus sp.	MGLP
2	AA, BA, CA, DA, G... (E, F, A, B, C...)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	24	Chemie (MPD)
3	EA, FA, GA, HA, IA... (A, B, C, D, E...)	water	water		18.0	904	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 250 (UniProt: P82597)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 196	Asp 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	177357.7
Non-Polymers*	Number of molecules	24
	Total formula weight	2836.2
All*	Total formula weight	180193.9

*Water molecules are not included.