4KE7

Crystal structure of Monoglyceride lipase from Bacillus sp. H257 in complex with an 1-myristoyl glycerol analogue

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Thermostable monoacylglycerol lipase	polymer	270	29560.6	2	UniProt (P82597) Pfam (PF12146)	Bacillus sp.	MGLP
2	C, E (A, B)	dodecyl hydrogen (S)-(3-azidopropyl)phosphonate	non-polymer		333.4	2	Chemie (1QX)
3	D, F (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
4	G, H (A, B)	water	water		18.0	408	Chemie (HOH)

Sequence modifications

A, B: 1 - 250 (UniProt: P82597)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59121.2
Non-Polymers*	Number of molecules	4
	Total formula weight	903.2
All*	Total formula weight	60024.4

*Water molecules are not included.