4KD4
Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Thermonuclease | polymer | 149 | 16843.3 | 1 | UniProt (P00644) Pfam (PF00565) In PDB | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease |
2 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
3 | A | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
4 | A | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 1 | Chemie (MRD) | |||
5 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
6 | water | water | 18.0 | 96 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: P00644)
PDB | External Database | Details |
---|---|---|
Leu 23 | Val 105 | engineered mutation |
Val 25 | Leu 107 | engineered mutation |
Ile 66 | Val 148 | engineered mutation |
Val 72 | Ile 154 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 16843.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 655.4 | |
All* | Total formula weight | 17498.7 |