4KB0

Crystal structure of RNase T in complex with a bluge DNA (Two nucleotide insertion CC )

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribonuclease T	polymer	235	25719.0	2	UniProt (P30014) Pfam (PF00929)	Escherichia coli	Exoribonuclease T, RNase T
2	C, D (C, D)	DNA (5'-D(*GP*GP*CP*CP*CP*TP*CP*TP*TP*TP*AP*GP*GP*GP*CP*CP*CP*C)-3')	polymer	18	5444.5	2
3	E, F, G, H (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	I, J, K, L (A, B, C, D)	water	water		18.0	423	Chemie (HOH)

Sequence modifications

A, B: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62327.1
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	62424.3

*Water molecules are not included.