4K9P

Crystal Structure of the His281Tyr/Ala460Ile Double Mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Benzoylformate decarboxylase	polymer	533	57167.5	4	UniProt (P20906) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Pseudomonas putida	BFD, BFDC
2	E, K, O, R (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	F, L, P, S (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
4	G, M (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	H, I, J, N, Q... (A, B, C, D)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
6	U, V, W, X (A, B, C, D)	water	water		18.0	917	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 528 (UniProt: P20906)

PDB	External Database	Details
Tyr 281	His 281	engineered mutation
Ile 460	Ala 460	engineered mutation
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228670.2
Non-Polymers*	Number of molecules	16
	Total formula weight	2462.7
All*	Total formula weight	231132.9

*Water molecules are not included.