4K9O

Crystal Structure of the Phe397Ala mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Benzoylformate decarboxylase	polymer	533	57024.3	4	UniProt (P20906)	Pseudomonas putida	BFD, BFDC
2	E, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	F, J, P, S (A, B, C, D)	THIAMINE DIPHOSPHATE	non-polymer		425.3	4	Chemie (TPP)
4	G, H, O, R (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5	K, L, M, N, Q... (B, C, D)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
6	U, V, W, X (A, B, C, D)	water	water		18.0	1309	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 528 (UniProt: P20906)

PDB	External Database	Details
Ala 397	Phe 397	engineered mutation
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228097.4
Non-Polymers*	Number of molecules	16
	Total formula weight	2462.7
All*	Total formula weight	230560.1

*Water molecules are not included.