4K9N

Crystal Structure of the Ala460Ile mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Benzoylformate decarboxylase	polymer	530	56817.1	4	UniProt (P20906)	Pseudomonas putida	BFD, BFDC
2	E, F, H, I, K... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
3	G, J, L, O (A, B, C, D)	2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL TRIHYDROGEN DIPHOSPHATE	non-polymer		440.3	4	Chemie (TZD)
4	M (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	P, Q, R, S (A, B, C, D)	water	water		18.0	1651	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 525 (UniProt: P20906)

PDB	External Database	Details
Ile 460	Ala 460	engineered mutation
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	227268.4
Non-Polymers*	Number of molecules	11
	Total formula weight	1999.1
All*	Total formula weight	229267.5

*Water molecules are not included.